Prestained Protein Ladder - Mid-range molecular weight (10 (2024)

FAQs

Prestained Protein Ladder - Mid-range molecular weight (10? ›

The Prestained protein ladder (mid-range molecular weight) a ready to use three-color (red, green, blue) protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. Each vial contains 500 µl volume, sufficient for 200-500 gels.

Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa.

Various manufacturers offer high molecular weight protein ladders for applications in protein separation and detection. These protein standards are comprised of a set of purified proteins of known molecular weight and tend to contain larger, heavier proteins. The upper limit of protein size can exceed 250 kDa.

The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed.

The Low Molecular Weight Protein Marker II for SDS electrophoresis is a liquid mixture of eight purified proteins for use in molecular weight estimation in the presence of the detergent sodium dodecyl sulphate (SDS).

Many high-molecular-mass (HMM) proteins (MW>100 kDa) are known to be involved in cytoskeleton, defence and immunity, transcription and translation in higher eukaryotic organisms.

Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored bands for easy identification.

However, an unstained protein ladder can only be visualized following staining with Coomassie or a similar non-specific protein stain. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel.

Low MW proteins – generally any protein with a MW less than 20 kDa – are vulnerable to poor resolution and poor retention. Therefore they are challenging to detect with standard SDS-PAGE and Western blotting protocols…but they are not impossible to detect, period.

Molecular weight markers or protein ladders are useful in estimating the size of proteins that have already been resolved by gel electrophoresis. They also aid in orienting the gel or membrane quickly and monitoring gel migration.

What is the purpose of the prestained standards? ›

Prestained standards allow direct visualization of the proteins' migration during electrophoresis and are useful to assess their subsequent transfer to membranes. Prestained standards can be used for size estimation, however unstained protein standards are recommended for the most accurate size determination.

Molecular weight markers, or ladders, are a set of standards that are used for determining the approximate size of a protein or a nucleic acid fragment run on an electrophoresis gel. These standards contain pre-determined fragment (or protein) sizes and concentrations.

Color-coded Prestained Protein Marker, Broad Range (10-250 kDa) is a mixture of purified proteins, covalently coupled to blue, green or orange dyes, that resolves to 11 bands between 10 and 250 kDa when subjected to electrophoresis. The protein concentrations are carefully balanced for even intensity.

Color-coded Prestained Protein Marker, High Range (43-315 kDa) is a mixture of purified proteins, covalently coupled to blue, green, orange, or pink dyes, that resolves to 8 bands between 43 and 315 kDa when electrophoresed. The protein concentrations are carefully balanced for even intensity.

If your total protein level is low, you may have a liver or kidney problem, or it may be that protein isn't being digested or absorbed properly. A high total protein level could indicate dehydration or a certain type of cancer, such as multiple myeloma, that causes protein to accumulate abnormally.

LMWHs were developed as anticoagulant/antithrombotic drugs since 1980s^3–7 and defined as heparin salts having an average molecular weight of less than 8000 Da and for which at least 60% of all chains have a molecular weight less than 8000 Da.

The Broad Range Protein Molecular Weight Markers consist of nine clearly identifiable bands at convenient molecular weights. The protein sizes are 10, 15, 25, 35, 50, 75, 100, 150 and 225kDa.

The standard consists of a wide range of molecular weight bands consisting of 12 proteins in the range of 3.5–260 kDa.

Precision Plus Protein Dual Xtra Standards are a mixture of 12 recombinant proteins (2–250 kD), including nine blue-stained bands and three pink reference bands (2, 25, and 75 kD). These standards can be detected by fluorescence when excited at red and green wavelengths.

Molecular weight markers, or ladders, are a set of standards that are used for determining the approximate size of a protein or a nucleic acid fragment run on an electrophoresis gel. These standards contain pre-determined fragment (or protein) sizes and concentrations.